Posted 20 February 2012 - 01:50 AM
So you need 3,000 cells in 190ul => 3,000/0.190 = 15,789cells/ml. = 1.57x10^4cell/ml
Now you say you have 2,000,000cells in 1ml (or 2x10^6cells/ml) so you just need to dilute them to the concentration you need:
Use C1V1=C2V2 where C1= 2x10^6cell/ml, V1= is the one you need to calculate, C2= 1.57x10^4cells/ml, V2= 18.24ml (190ulx96wells).
Another way to calculate this is by total number of cells you need: 3,000 cells per well in 96 wells = 288,000 = 2.88x10^5 cells So you need to take 288,000 cell from you initial 1ml (at 2x10^cells/ml) and then bring to the final volume for a 96 well plate.
I'd recommend to prepare a bit extra to make sure you have enough. So either calculate things for 100wells, or just round up to 20ml.
hope this helps.