1 reply to this topic

[aazmaish]

Hi,
I wonder can i re use a buffer in which i have added 5mM 2-mercaptoethanol (2-ME). I added 2-ME, 3 days ago,and i dont want to throw it away. Is it possible i add more 2-ME and re use it.

Thanks in advance

[bob1]

How did you store it? If at room temp, it is likely to have degraded, and you should throw it out. Remember the basic principle: If in doubt, throw it out. This rule stands because if you have something about which you are not sure (in your case the 2-ME), how will that uncertainty affect your results? At best, you would have to repeat the result completely with fresh buffer to be sure, and at worst, it would mean a complete failure of your experiment.