Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Culturing Human CD14+ monocytes

cell culture CD14+ human monocytes

  • Please log in to reply
No replies to this topic

#1 C.T

C.T

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 19 February 2012 - 10:24 AM

I have been having some problems with my monocyte cell cultures and wondered if anyone had any ideas.

I separate PBMCs from human blood using histopaque, then use miltenyi CD14 beads to get the CD14+ cells. After 48hr cell culture between 40-50% of my cells are dead.

I culture in RPMI supplemented with 10% FCS, L-glut and pen/strep, and have also tried adding 2-ME - with no benefit.

Any ideas to increase monoccyte cell survival?





Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.