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Buffer RWT for RNA extraction and on-column washes

Qiagene RNA microRNA Buffer RWT RNeasy

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#1 kaveh

kaveh

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Posted 19 February 2012 - 10:20 AM

Hi,

I use the Qiegen miRNeasy mini kit for extraction of total RNA and microRNAs from animal tissues. Due to a spill, I lost the RWT buffer component of the kit (used for the first wash on column). However, I still have other components (the columns, buffer RPE....etc) in exces. Does anyone know what else we could use for the first wash? I couldn't find the RWT recipe in the kit's catalog or their website.

many thanks.

K





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