Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

long term storage of cells at -80


  • Please log in to reply
1 reply to this topic

#1 sara.r

sara.r

    sara.r

  • Active Members
  • PipPipPipPipPip
  • 86 posts
1
Neutral

Posted 19 February 2012 - 03:43 AM

Hi every one,

I stored some cells in 90% FBS cell freezing medium at -80 freezer over a year ago. I revived them recently, cell viability was 90% and cell growth was normal. we always transfer friezed cells to -180 for long term storage. does any one know if cell characteristics will stay constant after long time storage at -80?

Bests

sara

#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,674 posts
396
Excellent

Posted 19 February 2012 - 12:21 PM

Cells should not be stored long term at -80 as they do slowly die off. It will depend on which cell line you are using as to how long they will last in the -80, but most cell lines will not last much more than a year at -80. There are apparently still some enzymatic processes that can continue at -80 which reduce the viability of the cells. Ice crystal formation, which is the biggest killer of frozen cells also is more likely to occur.

look at it this way - the 10% of your cells that didn't survive - which 10% were they? Could you be artificially selecting for a cell population that can survive this particular stress? How would this selection affect your results? how similar are these cells now to the parent line?




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.