So prior to today, I've only been using the Invitrogen Miniprep kit. Using that protocol I start with ~5-6mL bacterial culture and spin the WHOLE thing down and work with that. I'm missing one or two things to proceed with this kit anymore, so now I'm movinng on to our Promega PureYield kit.
This one, however initially says to start with 600uL of culture, and that alternatively, 3mL of culture can be used for higher yields.
Being that I was "nestled" on always using 6mL cultures completely, I'm not sure what I should do. Does anybody here have experience of using more culture than what these protocols specify for? I want as much DNA as possible, but I don't want to run two collomns for each culture, really.
Edited by byobortolazzo, 18 February 2012 - 12:32 PM.