Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

plasmid extraction

gel electrophoresis

  • Please log in to reply
3 replies to this topic

#1 hidayahwannur

hidayahwannur

    member

  • Active Members
  • Pip
  • 22 posts
0
Neutral

Posted 17 February 2012 - 08:45 PM

result plasmid extraction 120212.jpg result plasmid extraction 120212.jpg

#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,831 posts
415
Excellent

Posted 18 February 2012 - 04:11 PM

What is your question? It could be plasmids, but what size are you expecting? what are the sizes on your ladders (I think you have two on there)?

#3 hidayahwannur

hidayahwannur

    member

  • Active Members
  • Pip
  • 22 posts
0
Neutral

Posted 18 February 2012 - 05:29 PM

i expect there will be smooth, clear and nice band appear..but it looks like a bad result, right? i use 1kb DNA and 100bp DNA as ladder

#4 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,831 posts
415
Excellent

Posted 20 February 2012 - 03:07 PM

Plasmids typically have multiple bands - corresponding to the different forms you find plasmids in, supercoiled, nicked, and linear. You may see all of these or only a subset depending on howgood you are at preparing the DNA.

How big are you expecting your plasmids to be?





Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.