3 replies to this topic

[Luna2012]

Hi all,

I have to sonicate c. elegans for a Genomic DNA prep. The protocol asks for for 30 pulses at a 20% amplitude. I'm using a Fisher Sonicator Dismembrator model 100, and I'm not sure how to set it up to 20% amplitude. There's a knob (0-10) but I would like to know which number correspond to 20% amplitude...


Thanks for any help or suggestion
:-)

[ickypimp]

Read the manual...

[Luna2012]

there's no manual!!! that was the first thing I looked for... Otherwise I would not been asking!!!!

[phage434]

Unfortunately, there is no easy or standard way to quantitate sonication conditions.  Every sonicator is different.  It probably doesn't make a lot of difference, as long as you sonicate long enough and hard enough, and keep the suspension cool.  I'd start with a low setting and alternate soncation and putting the worms on ice, perhaps 30-60 seconds of sonication and 2-3 minutes on ice.

Some sonicators now report the wattage of the delivered ultrasound, which really is the important number, but very few do this.