Posted 16 February 2012 - 10:48 AM
sorry for my English...
I have a really big problem with PFGE, we use a Biorad chef mapper3
we tried to run a low range pfge ladder (neb) and a lamda ladder (neb) but the result is on this image...
the running buffer is 0.5 TBE.
Thnx for your help!
Posted 16 February 2012 - 11:04 AM
I don't understand why people expect us to guess these critical parameters when they ask for help.
Posted 16 February 2012 - 11:41 AM
Sorry...but I thought it is not important. Thnx again
Posted 16 February 2012 - 05:46 PM
As a comparison point, look at the gel running conditions for the NEB low range PFG ladder. 6 v/cm, 1% gel, 15C, but switching times from 1-12 seconds, run for 15 hours.
Posted 17 February 2012 - 01:57 AM
I suppose the problem is on chef mapper, I have runned the gel several times in 2011 with very good images with 2,2-63,8 etc. I checked agarose, TBE buffer and seems they are ok.
Here you can see an image from 11/2011. the first and the last lanes are low range ladder from NEB, the same I use every time.
Possibly I must contact Biorad- service