My goal is to confirm the results of the genome microarray experiments that were done by someone else. This is for rat spinal cord RNA expression.
To start I selected an internal standard, based on the literature I picked a ribosomal RNA, Rpl27. I used genbank to identify the sequence and came up with
NM_022514. I put that into PrimerQuest (primer design tool available on Integrated DNA Technologies website). That gave me 5 primer sets and I ordered all 5 and none of them gave me a band.