I am a very new lab tech with little training, so this question will most likely seem a little silly to most:
I am using a DNA extraction protocol which requires the addition 20 ul of 30ug/ml DNAseI (Roche) to 750ul of template. (then 30min incubation at 37C)
I ordered DNAseI and it came with a concentration of 1u/ul (1000units), and also came with reaction buffer and EDTA.
I've been reading about DNAseI and its use and such, but am still confused as to how get the DNAseI to be a concentration of 30ug/ml from 1u/ul. Am I supposed to be mixing the DNAseI with the reaction buffer to make it a concentration of 30ug/ml?
Like I said, kind of a silly question, but all of this stuff is so new to me!
Thanks for any help.
Edited by newtogenetics, 15 February 2012 - 02:35 PM.