RT-PCR in a single tube
Posted 15 February 2012 - 08:30 AM
Starting from purified RNA...is it possible to perform a retrotranscription followed by a PCR amplification in a single tube? What I mean is whether it is possible to have a mix of a retrotranscriptase and a Taq-polymerase in a single buffer compatible with both enzymes and to run the RT program at -let´s say- 37ºC and to follow with the PCR program.
If the answer were "yes", I would appreciate any references or commercial product names.
Thank you all in advance for your kind answers!
Posted 16 February 2012 - 08:15 AM
Our country has a serious deficiency in lighthouses. I assume the main reason is that we have no sea.
I never trust anything that can't be doubted.
'Normal' is a dryer setting. - Elizabeth Moon
Posted 16 February 2012 - 08:21 AM