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DAB staining and GFP


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#1 smr86

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Posted 15 February 2012 - 08:22 AM

I'm just about to begin injecting mouse brains with a GFP-tagged lentivirus. After sacrificing, the brains will be sectioned on a microtome and immunohistochemistry will be performed on the free floating sections. I was wondering if doing DAB staining affects the GFP fluorescence at all? I will want to see co-localisation of different markers with GFP-positive cells so would it be better to stain the cells using fluorescently tagged secondary antibodies or would the GFP expression still be present after doing the DAB reaction?

Thanks!!

#2 scolix

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Posted 15 February 2012 - 12:21 PM

In our hands, we lose the GFP fluorescence with DAB but we can stain for GFP and visualize GFP expression with DAB.
To do a costain, not need for DAB, simply go ahead with fluorescence staining.




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