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[smr86]

I'm just about to begin injecting mouse brains with a GFP-tagged lentivirus. After sacrificing, the brains will be sectioned on a microtome and immunohistochemistry will be performed on the free floating sections.  I was wondering if doing DAB staining affects the GFP fluorescence at all?  I will want to see co-localisation of different markers with GFP-positive cells so would it be better to stain the cells using fluorescently tagged secondary antibodies or would the GFP expression still be present after doing the DAB reaction?

Thanks!!

[scolix]

In our hands, we lose the GFP fluorescence with DAB but we can stain for GFP and visualize GFP expression with DAB.
To do a costain, not need for DAB, simply go ahead with fluorescence staining.