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why Phusion polymerase is not recommended for overlap PCR?
1 reply to this topic
Posted 15 February 2012 - 08:06 AM
According to this protocol it is not recommended to use Phusion polymerase in overlap PCR reactions (4.2), Why?
Posted 15 February 2012 - 05:38 PM
Not sure why they say that, a previous PhD student in our lab used Phusion for her SOE PCR and it worked just fine.