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why Phusion polymerase is not recommended for overlap PCR?

Started by Curtis, Feb 15 2012 08:06 AM

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#1 Curtis

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Posted 15 February 2012 - 08:06 AM

According to this protocol it is not recommended to use Phusion polymerase in overlap PCR reactions (4.2), Why?

http://openwetware.o...erlap_Extension

#2 leelee

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Posted 15 February 2012 - 05:38 PM

Not sure why they say that, a previous PhD student in our lab used Phusion for her SOE PCR and it worked just fine.
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