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Detection of glycosylations on Western blotted proteins


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#1 Inmost sun

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Posted 15 February 2012 - 07:00 AM

what are useful tools (lectins?) to detect N-glycosylations or glycosulphates on Western blot?

#2 mdfenko

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Posted 15 February 2012 - 09:17 AM

i used concanavilin a and horse radish peroxidase in a procedure to stain gels for glycoproteins. it can be adapted for blots (i did).
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#3 Inmost sun

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Posted 16 February 2012 - 06:48 AM

thank you mdfenko for reliable answerPosted Image




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