Is Kozak's full sequence necessary? or just ACCATG is enough?
Posted 14 February 2012 - 07:16 PM
Posted 15 February 2012 - 12:09 PM
Posted 15 February 2012 - 01:45 PM
Posted 27 February 2012 - 04:39 AM
I do apologise this reply is late. I realize that I had not replied your email.
A KOZAK sequence is not absolutely essential for expression- it enhances expression in some cases. The pCI-neo has CMV promoter and an intron upstream of MCS, so I would expect a cDNA with a ATG start site to work well. Regarding the distance between the ATG and promoter, that is already taken care of by the intron found on the plasmid.
I hope this helps!
Posted 27 February 2012 - 12:35 PM