Hi everyone.
I'm pretty new to cell culture work and am trying to work something out.
I want to seed HaCats into 8-well culture/chamber slides (http://www.emsdiasum...on/chamber.aspx)
After 72 hours I want to harvest them for immunofluorescent staining.
I am wondering how many cells I should put into each well so that they are essentially confluent after 72 hours
Any ideas?
I think the area in each well is ~0.8-1cm2
Thankyou for your time
thommo
0
Hacat Seeding into 8-well chamber slide
Started by thommo, Feb 13 2012 07:08 PM
hacat seed chamber slide
1 reply to this topic
#2
bob1
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Thelymitra pulchella
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Excellent
Excellent
Posted 14 February 2012 - 01:11 PM
It depends a bit on how big HaCats are and how fast they grow, but at confluency you will have a little bit less than 105 cells in each well. If you know how fast they grow roughly, you should be able to work out about how many to seed. Assuming a doubling time of approx 24 hours, you probably would want about 10000 per well to start.
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