3 replies to this topic

[joy123]

Hi, I want to make some how to store LB-agar-chloramphenicol-X-gal-IPTG plate, but I don't know how should I store them and how long it can last.

Would you please give me some suggestions?

Thanks a lot!

[bob1]

Store at 4 deg C sealed (in a bag is fine) to prevent water loss.  The X-gal will degrade relatively fast, I think about 2-3 weeks will be the maximum.

[joy123]

bob1, on 13 February 2012 - 02:03 PM, said:

Store at 4 deg C sealed (in a bag is fine) to prevent water loss.  The X-gal will degrade relatively fast, I think about 2-3 weeks will be the maximum.

Thanks very much for your advice!

[phage434]

You may find S-Gal (from Sigma) much easier to use for differentiating bla+ strains.  It's a straight substitute for X-Gal, and I think it works a lot better.