Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

how to store LB-agar-antibiotic- X-gal-IPTG plate

LB plate store

  • Please log in to reply
3 replies to this topic

#1 joy123

joy123

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 105 posts
3
Neutral

Posted 13 February 2012 - 01:22 PM

Hi, I want to make some how to store LB-agar-chloramphenicol-X-gal-IPTG plate, but I don't know how should I store them and how long it can last.

Would you please give me some suggestions?

Thanks a lot!

#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,513 posts
371
Excellent

Posted 13 February 2012 - 02:03 PM

Store at 4 deg C sealed (in a bag is fine) to prevent water loss. The X-gal will degrade relatively fast, I think about 2-3 weeks will be the maximum.

#3 joy123

joy123

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 105 posts
3
Neutral

Posted 13 February 2012 - 02:13 PM

Store at 4 deg C sealed (in a bag is fine) to prevent water loss. The X-gal will degrade relatively fast, I think about 2-3 weeks will be the maximum.

Thanks very much for your advice!

#4 phage434

phage434

    Veteran

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 2,376 posts
227
Excellent

Posted 13 February 2012 - 08:37 PM

You may find S-Gal (from Sigma) much easier to use for differentiating bla+ strains. It's a straight substitute for X-Gal, and I think it works a lot better.





Also tagged with one or more of these keywords: LB plate, store

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.