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histone proteins extraction and 2D


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#1 pakbiochemist

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Posted 13 February 2012 - 07:07 AM

Dear Friendz

i am working on histone proteins. Plz suggest if i can use an extraction protocol designed on cell lines for tissue samples. How much minimum sample i can load on 2D since i have shortage of protein samples.

If anyone working on mamalian histone prorteins plz contact me i need to clear some confusions about these proteins


Binsan

#2 Inmost sun

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Posted 28 February 2012 - 03:30 AM

amount depends on your system; for mini gel 2D, strips can be loaded up to 100 µg




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