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problems with Bisulfite PCR


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#1 vaidhi

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Posted 12 February 2012 - 09:40 PM

Hello,

I'm new to the the world of methylation and am trying to amplify CpG islands after bisulfite conversion. But when i run the PCR products on a gel i get smears and they aren't even in the correct range. I designed my primers using meth primer and when the first PCR didn't work i tried lowering the extension temperature to 63 C ( first i used 72 C).

Any suggestions would be greatly appreciated.

Edited by vaidhi, 12 February 2012 - 09:41 PM.


#2 methylnick

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Posted 29 March 2012 - 02:03 PM

most liekly a primer design problem, I would try to redesign them again, maybe using MethylPrimer Express which is freely available.




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