Problem with transwell migrationtranswell migration
Posted 12 February 2012 - 06:43 PM
I am new in doing migration assay. I have problem maintain the cell viability after 3 hr of migration. I only get 8% to 12% cell that are alive after migration. I used 0.5 BSA migration medium and 3% FBS migration medium and FBS give me just a little better viability. I just don't understand how they all die after just 3hr of migration. Can someone give me some tips on migration assay please?
Thanks in advance!
Posted 17 February 2012 - 06:57 AM
Posted 17 February 2012 - 09:59 AM
Posted 17 February 2012 - 09:57 PM
Posted 16 October 2012 - 01:11 AM
How do you know they are dead? Did you check if they are still ABOVE the chamber? perhaps they are still clumps and can"t pass through Transwell?