4 replies to this topic

[yi198720022004]

Hello,

I am new in doing migration assay. I have problem maintain the cell viability after 3 hr of migration. I only get 8% to 12% cell that are alive after migration. I used 0.5 BSA migration medium and 3% FBS migration medium and FBS give me just a little better viability. I just don't understand how they all die after just 3hr of migration. Can someone give me some tips on migration assay please?

Thanks in advance!

[Inmost sun]

I suppose you work with adherent cells, you should over coated wells; which FBS % do you use in normal cell culture?

[scolix]

some cells are not suitable for migration. Has this cell line been described in literature for these experiments.

[yi198720022004]

I am working with black 6 mouse splenoytes. I did total splenocytes migration and I am looking specifically for Th17 subset. I used 10%FBS for normal cell culture. I read it somewhere says migration assay should avoid FBS becuase the cell will migration nonspecifically that's why I used BSA but they died.

[wernerlael]

How do you know they are dead?
Did you check if they are still ABOVE the chamber?
perhaps they are still clumps and can"t pass through Transwell?