Making BL21 competent cells
Posted 12 February 2012 - 01:33 AM
I'm making competent cells for the first time and I have to use 20mM Mg2SO4 and 10mM KCl so I'm just wondering how to make these solutions. I assume I make 1M solution of each and to do so I would do the following:
To make 1M Mg2SO4 I would dissolve 144.673g of Mg2SO4 in 1000mL of water but seeing as thats too much if I wanted to 100mL I would dissolve 14.5g in 100mL of water?..Is this correct?
To make 1M of KCl I would dissolve 74.55g in 1000mLs of water but once again 1000mLs is too much so if I wanted to make 100mL i would dissolve 7.4g in 100mLs of water...is this correct?
Posted 12 February 2012 - 01:10 PM
Posted 14 February 2012 - 08:02 AM
genius does what it must
i do what i get paid to do