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Question on Chromatin Isolation by Fractionation (Mendez/Stillman protocol)

Mendez and Stillman Fractionation chromatin isolation protocol

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#1 angelawu

angelawu

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Posted 10 February 2012 - 04:45 PM

Hello,


This is my first time using the Mendez Stillman protocol to isolate chromatin via fractionation (usually for ChIP, I just lyse and shear away...), so this may be a dumb questions. But looking through the Mendez protocol, it appears that they tell you to keep supernatant fraction S2 (the supernatant left after centrifuging at 20,000g for 5 mins), but don't do anything with it later on. I just want to know, why S2 is kept, and what I am supposed to do with it?

Thanks!

(I attached the protocol for ease of reference)

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Also tagged with one or more of these keywords: Mendez and Stillman, Fractionation, chromatin, isolation, protocol

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