cell plating density
Posted 10 February 2012 - 01:04 PM
I am kind of confused with the number of cells needed to plate in a well plate..
data i have:
cell/ mL : 6.175 X 10^5
suspension volume (Trypsin + FBS) : 4 mL
I need to add 100 micro L of 10 ^ 5 cell/mL for the above.. how do i do that?
If i need to add 5 X 10^4 cells per well in a well plate..how do i do it?
please respond ASAP
Posted 10 February 2012 - 10:25 PM
Dilute the cell suspension by 6 fold (1 ml of stock + 5 ml media should do it) and you should have the right density. and add 100ul into each well.
Does this make sense?
Posted 14 February 2012 - 02:47 PM