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cell plating density


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2 replies to this topic

#1 mehtashah

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Posted 10 February 2012 - 01:04 PM

hi everyone,
I am kind of confused with the number of cells needed to plate in a well plate..
data i have:
cell/ mL : 6.175 X 10^5
suspension volume (Trypsin + FBS) : 4 mL
I need to add 100 micro L of 10 ^ 5 cell/mL for the above.. how do i do that?
or
If i need to add 5 X 10^4 cells per well in a well plate..how do i do it?

please respond ASAP

Regards.

#2 scolix

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Posted 10 February 2012 - 10:25 PM

you have 6x 10^5cells/ml and you need 1x 10^5cells/ ml cells, so your stock is 6 fold concentrated.

Dilute the cell suspension by 6 fold (1 ml of stock + 5 ml media should do it) and you should have the right density. and add 100ul into each well.

Does this make sense?

#3 mehtashah

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Posted 14 February 2012 - 02:47 PM

yup...i got it.... have done it already :) thanx for the reply




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