What kind of cell culture contamination is it?queer cell culture
Posted 10 February 2012 - 10:19 AM
I need help to identify what kind of contamination happened to my cell culture. I have HEK293 just thawed from liquid nitrogen and split just once. Then I found this contamination:
When I swirled around the culture plate, a puff of powder-like or dust-like things were blew up from the bottom of culture plate and then sink down. It could be observed directly by eyes. This cell culture contaminator feels and looks a lot like the bacteria culture flask: when you swirl the bacterial culture flask, the cultured bacteria are blasted up.
1) medium is not turbid, still clear and with normal red or purple color (PH normal). (so , it is not bacteria contamination?)
2) cells are still attached well. no obvious cell debris floating in the medium. Checking under light microscopy, cells have normal morphology.
3) The contaminators did not have the shapes of thread, rod or branches. Anti-fungus agent not help either.
So, anyone has any ideas? Many thanks for your time.
Posted 10 February 2012 - 12:11 PM
It was fine a day before and happened later. Definitely not from Media.
Posted 10 February 2012 - 10:30 PM
I haven't encountered some thing like this before.
Posted 11 February 2012 - 09:52 AM
Posted 13 February 2012 - 06:57 AM
Posted 13 February 2012 - 02:20 PM
Posted 15 February 2012 - 12:38 PM
It is unlikely due to frequently freeze/thaw cylce 'cause this dust-like problem came back again to the newly spit cells. This is what I did: wash with PBS twice-trypsinize- add serum-containing growth medium to stop trypsin- then directly seed to a new flask. With help, now, I could upload some pics, taken with 10X objective. One of them (forgot which one, ) is off focused and trying to take a snap at those floating unknown, Please suggest. Thanks!
Posted 15 February 2012 - 01:50 PM