western blot loading control
Posted 10 February 2012 - 06:04 AM
Posted 11 February 2012 - 10:25 AM
It sounds more like you are having specificity issues with the antibodies, which are probably not designed for yeast work - try titrating the antibodies out to minimise non-specific binding. You could also work try more stringent conditions - higher salt and/or more detergents in the antibody incubation and wash steps.
Posted 14 February 2012 - 12:36 PM
you may also be looking at non-specific staining of a common artifact which appears in the 50s-60s range (may also be in the 40s).
genius does what it must
i do what i get paid to do