Help. Lentivirus. Good titer but no transduction
Posted 10 February 2012 - 03:41 AM
Anybody can help me????????? Please I do not what can I do.
Posted 10 February 2012 - 11:07 AM
Have a positive control for the transduction.
How long have you incubated cells with virus?
Posted 15 February 2012 - 02:51 AM
I have incubated 24 h and 48 h with the virus and I have tried to be the expression 1,2,3,4,5 days after the infections.
Thank you very much!
Posted 15 February 2012 - 12:13 PM
Wait for up to 1 week with neuronal cultures. A lot depends on the promoter too. It can take longer if the promoter is a very weak one.