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Nickel Column Purficiation


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#1 Smog187

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Posted 09 February 2012 - 12:36 PM

We are having problems with the Imidazole that we use for elution in our Nickel Column. We believe that the Imidazole is tearing up our protein. Is there anything else I can use to help elute the Protein, instead of using Imidazole? BTW our protein has a 6X-His tag.

#2 mdfenko

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Posted 14 February 2012 - 01:09 PM

you may be able to elute with edta (it's used during regeneration)
talent does what it can
genius does what it must
i do what i get paid to do

#3 Bluefunk

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Posted 16 February 2012 - 07:58 PM

If thats true i would just try another tag. Strep? Flag?




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