Frozen vs paraffin: Confocal microscopy
Posted 09 February 2012 - 06:21 AM
I was wondering if anyone knows which tissue preperation (frozen vs paraffin) is better for confocal microscopy. Also, I will be using antibodies against phospho-proteins and I have heard that these do no do so well when tissue has been embedded in paraffin.
Any insight would be greatly appreciated!
- cdomi018 likes this
Posted 09 February 2012 - 11:01 AM
I have no experience comparing phospho antibodies on sections.
Posted 09 February 2012 - 11:49 AM
Il dort. Quoique le sort fût pour lui bien étrange,
Il vivait. Il mourut quand il n'eut plus son ange;
La chose simplement d'elle-même arriva,
Comme la nuit se fait lorsque le jour s'en va.
Posted 13 February 2012 - 09:25 AM