I am trying to harvest the bone marrow and induce the growth of BMDM. However, I have came across many protocols that keep emphasizing the use of non-tissue culture plates (bacterial petri dishes) instead of tissue culture plates to grow them, while other seem to be ok using tissue culture plates.
What difference does it make if I use tissue culture plates instead of the non-tissue culture plate? Is this to eliminate the adherence of fibroblasts or DC ? isnt the addition of m-CSF is good enough to ensure only macrophages will grow by the end of the 6th day? any suggestions on what to use??
petri dishes vs tissue culture plates to grow BMDM
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