hi everyone,
I am trying to harvest the bone marrow and induce the growth of BMDM. However, I have came across many protocols that keep emphasizing the use of non-tissue culture plates (bacterial petri dishes) instead of tissue culture plates to grow them, while other seem to be ok using tissue culture plates.
What difference does it make if I use tissue culture plates instead of the non-tissue culture plate? Is this to eliminate the adherence of fibroblasts or DC ? isnt the addition of m-CSF is good enough to ensure only macrophages will grow by the end of the 6th day? any suggestions on what to use??
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