Hello everybody,
I just want to ask about some calculations about varying concentrations of drug treatments to my cells. For example, i have a 1uM stock of the drug and I need to treat the cells (3x10^3) in 3 cm dish with 100 nM in 2 ml of medium.
Does that mean i just simply dilute my drug to 100 nM from my 1uM stock and add it directly to my cells? or do i need to compute and consider the 2 ml of medium in the dish?
if you can share with me links or books to do math and computations of concentrations, i would be happy to know. thank yoou...
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2 replies to this topic
#2
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a student
Posted 09 February 2012 - 10:18 AM
You have a stock of 1uM and you need 100nM. SO basically dilution factor of 10. For 2ml, 2ml/10 = 0.2ml. SO you need to add 200ul of the stock + 1800ul of media, for final concentration of 100nM.
There is the easier way below.
http://www.graphpad....olarityform.cfm
Go to the bottom, to dilute a stock solution. Enter the values and final volume as 2ml, and you will have the dilution factor.
There is the easier way below.
http://www.graphpad....olarityform.cfm
Go to the bottom, to dilute a stock solution. Enter the values and final volume as 2ml, and you will have the dilution factor.
#3
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- Active Members
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- 3,047 posts
Hmmm, I think it's working
Posted 09 February 2012 - 12:49 PM
soymilk14, on 08 February 2012 - 06:53 PM, said:
Hello everybody,
I just want to ask about some calculations about varying concentrations of drug treatments to my cells. For example, i have a 1uM stock of the drug and I need to treat the cells (3x10^3) in 3 cm dish with 100 nM in 2 ml of medium.
Does that mean i just simply dilute my drug to 100 nM from my 1uM stock and add it directly to my cells? or do i need to compute and consider the 2 ml of medium in the dish?
if you can share with me links or books to do math and computations of concentrations, i would be happy to know. thank yoou...
I just want to ask about some calculations about varying concentrations of drug treatments to my cells. For example, i have a 1uM stock of the drug and I need to treat the cells (3x10^3) in 3 cm dish with 100 nM in 2 ml of medium.
Does that mean i just simply dilute my drug to 100 nM from my 1uM stock and add it directly to my cells? or do i need to compute and consider the 2 ml of medium in the dish?
if you can share with me links or books to do math and computations of concentrations, i would be happy to know. thank yoou...
Note that concentration of solutions is entirely independent of volume - whether you have 100 ul or 10000 l you will still have a [defined concentration] solution! From your post I can't tell if you need to add exactly 100 nm to each treatment or whether you want a 100 nm/l solution - in which case Scolix has answered you problem
