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to treat cells with inhibitor


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#1 soymilk14

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Posted 08 February 2012 - 06:53 PM

Hello everybody,

I just want to ask about some calculations about varying concentrations of drug treatments to my cells. For example, i have a 1uM stock of the drug and I need to treat the cells (3x10^3) in 3 cm dish with 100 nM in 2 ml of medium.

Does that mean i just simply dilute my drug to 100 nM from my 1uM stock and add it directly to my cells? or do i need to compute and consider the 2 ml of medium in the dish?

if you can share with me links or books to do math and computations of concentrations, i would be happy to know. thank yoou...

#2 scolix

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Posted 09 February 2012 - 10:18 AM

You have a stock of 1uM and you need 100nM. SO basically dilution factor of 10. For 2ml, 2ml/10 = 0.2ml. SO you need to add 200ul of the stock + 1800ul of media, for final concentration of 100nM.

There is the easier way below.

http://www.graphpad....olarityform.cfm

Go to the bottom, to dilute a stock solution. Enter the values and final volume as 2ml, and you will have the dilution factor.

#3 bob1

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Posted 09 February 2012 - 12:49 PM

Hello everybody,

I just want to ask about some calculations about varying concentrations of drug treatments to my cells. For example, i have a 1uM stock of the drug and I need to treat the cells (3x10^3) in 3 cm dish with 100 nM in 2 ml of medium.

Does that mean i just simply dilute my drug to 100 nM from my 1uM stock and add it directly to my cells? or do i need to compute and consider the 2 ml of medium in the dish?

if you can share with me links or books to do math and computations of concentrations, i would be happy to know. thank yoou...

Note that concentration of solutions is entirely independent of volume - whether you have 100 ul or 10000 l you will still have a [defined concentration] solution! From your post I can't tell if you need to add exactly 100 nm to each treatment or whether you want a 100 nm/l solution - in which case Scolix has answered you problem




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