Hi,
Could anyone please tell me what concentration of PI I should use to stain dead cells before running FACS? It seems to vary from protocol to protocol... My stock concentration is 1mg/ml.
Thanks!
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scolix
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Posted 09 February 2012 - 10:22 AM
Check the data sheet for PI. And try out a couple of conc. above and below the recommended. We had problems with each supplier of PI and it had to be titrated.
Also tagged with one or more of these keywords: FACS, dead cells, PI
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