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Propidium iodide staining

FACS dead cells PI

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#1 sc_queen

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Posted 08 February 2012 - 05:03 PM

Hi,

Could anyone please tell me what concentration of PI I should use to stain dead cells before running FACS? It seems to vary from protocol to protocol... My stock concentration is 1mg/ml.

Thanks!

#2 scolix

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Posted 09 February 2012 - 10:22 AM

Check the data sheet for PI. And try out a couple of conc. above and below the recommended. We had problems with each supplier of PI and it had to be titrated.





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