Dear all, I want to use 3t3L1 cells to work on differenciated adipocytes:
Different protocols are available:
I have some questions concerning different steps:
can I use FBS for proliferation instead of calf serum?
Is it necessary to use insulin in differenciated adipocyte maintenance medium (used 2 days after the insulin media and 4 days after differentiation initiation)?
Thank you very much.
Best regards,
Fred
Submit your paper to J Biol Methods today!

3T3-L1 differenciation and maintenance
Started by fredUNH, Feb 08 2012 03:12 AM
adipocyte differenc cell culture insulin 3t3-l1 3t3
4 replies to this topic
#1
Posted 08 February 2012 - 03:12 AM
#2
Posted 08 February 2012 - 03:51 PM
FBS (foetal bovine serum)= foetal calf serum, just a name change to reflect the source, as other animals also have calves.
#3
Posted 09 February 2012 - 12:07 AM
Thank you, but I think that serum from newborn calf is also available and used fir this cell model. I knew that calf and bovine was the same.
#4
Posted 09 February 2012 - 05:19 AM
Hey, we normally use 10% FBS-supplemented DMEM all the way from undifferentiated 3t3-l1 fibroblasts to mature adipocytes. In the past we have also used DMEM-F12 supplemented with FBS or calf serum for preadipocytes. All of them work just fine.
We also use standard differentiation medium (IBMX, ins, dex) for 2 days, then another 2 days w/ insulin-supplemented medium and finally we go back to the 10% FBS/DMEM (no insulin).
We get cute and round adipocytes with this method.
www.adipofat.com
We also use standard differentiation medium (IBMX, ins, dex) for 2 days, then another 2 days w/ insulin-supplemented medium and finally we go back to the 10% FBS/DMEM (no insulin).
We get cute and round adipocytes with this method.
www.adipofat.com
#5
Posted 09 February 2012 - 06:45 AM
Thank you very much AdipoFat, your message corresponds to what i generally see.!
Also tagged with one or more of these keywords: adipocyte differenc, cell culture, insulin, 3t3-l1, 3t3
![]() |
Protocols and Techniques Forums →
Cell Biology →
Should Lineage marker cocktail be used in cell lines to remove lineage specificStarted by rv1234, 18 Apr 2018 ![]() |
|
![]() |
|
![]() |
Protocols and Techniques Forums →
General Lab Techniques →
Beads coated with integrins?Started by EvaG, 28 Mar 2018 ![]() |
|
![]() |
|
Protocols and Techniques Forums →
Tissue and Cell Culture →
Counting number of cellsStarted by Mad Researcher, 25 Dec 2017 ![]() |
|
![]() |
||
Protocols and Techniques Forums →
Tissue and Cell Culture →
Determine maximal passage numberStarted by My.Cyanide, 06 Sep 2017 ![]() |
|
![]() |
||
Protocols and Techniques Forums →
Cell Biology →
What is the best dopaminergic cell line for a in-vitro parkinson's disease mStarted by Katlnz, 12 Jul 2017 ![]() |
|
![]() |