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[hanna12345]

I asked this question in another forum, and I'm trying also here:

I want to stain primery cells in suspension for cofocal microscopy.
(until today I had no problems with immunostaining cell which were grown on coverslips, but now it's more complicated situation because the cells are much more sensitive, and I think that all the process of fixation (formAl. 3%), permeabilization(triton-X), 1',2' Ab , DAPI and washes between the steps and spindowns over and over again will kill all my cells even if I begin with a lot of them.)

I there anybody who tried to do it?

help?

thanks
Hanna