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[Monika Gluch-Lutwin]

Hi,



I have a problem with expression of GABA receptor. Nowadays I am working with WSS-1 cell line form ATCC. Unfortunately I have problem with obtaining high expression of β-receptors in this cell line. The cell were cultured accordingly with the recommendations of ATCC. Vibality of cell after  a passage were about 98%. In addition, lead the culture of cell with Geneticin (400µg/ml). But also we don’t observed high expression of β-receptors in this cell line (the Geneticin was added to 2 passage of cells). Cells cultured in medium with Geneticin showed smaller increase compared to cells in medium without geneticin. After 2 days of culture reached confluency less.  Has anyone worked with this cell line? This is very important for me.