No replies to this topic

[fighter]

Hello all,
I have to over express quite a few genes in Arabidopsis. I am planning to go ahead with cDNA of gene. But I am little worried if I will be in trouble because some genes has some regulatory sequences in introns as well. So is it safe to use genomic DNA? Anybody faced any problem by using cDNA ? Please let me know if you can help with some suggestions. Thanks in advance.....

Edited by fighter, 07 February 2012 - 01:25 AM.