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RNA isolation- can't get pellet after isopropyl

RNA isolation

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#1 lqhstephanie

lqhstephanie

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Posted 06 February 2012 - 01:54 PM

I followed the TRIzol protocol. But I could get any pellet after adding isopropyl. And I removed most of the liquid anyway thinking that maybe I just didn't see it clearly and added 75% EtOH. Still no pellet (I vortex. Then I stopped there and just stored the whole thing into -20C . I don't know what to do next.

It was my first time doing RNA isolation. :(





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