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Reverse Transcription PCR Problem with Primer pair


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#1 ElenaSak



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Posted 06 February 2012 - 12:45 PM

I started recently to study the co-transcription of some catabolic genes in a gram positive bacterium with Reverse-Transcription PCR. The primer pairs which i designed seems to work good though the bands are not very intense.
Before i use the primers in RT-PCR i checked them in genomic DNA and all of them gave the desirible products.The problem is one pair which although gives one band with genomic DNA, in RT-PCR gives two bands.
I note that i used RobusT RT-PCR kit for Reverse-Transcription.
If anyone has an idea for what is happening, i would like to know...


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