I would like to try co-expression of two genes in one plasmid under different promoters.
I'm looking to pFast bac Dual plasmid then I've seen two promoter p10 and pPH with two MCS, and they are at opposite direction.
I don't know if the expression will be into two directions. I mean how to choose the restriction enzymes (which one, forward and reverse with the other promoter) they seem to me in opposition.
Could you please help.
Edited by saad, 06 February 2012 - 07:13 AM.