Hello,
Recently I started having problems with my LNA ISH on the rat brain. First the ISH worked fine. But all of a sudden I started having problems. Mir124 should be expressed throughout the whole brain. This is what I saw during my first LNA ISHs. However, the last couple of times there are only parts of the brain that show expression of mir124. The staining looks really spotty. I have already refreshed all buffers and tried new probe. Nothing works... Any suggestions what might be the problem?
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