why my solution II turns cloudy during plasmid extraction?
Posted 04 February 2012 - 10:11 AM
Posted 04 February 2012 - 10:45 AM
Posted 04 February 2012 - 01:09 PM
You should either make the solution II fresh each time (best), or store it with the lid tightly closed to prevent the NaOH from absorbing CO2 from the atmosphere and altering the pH.
Posted 06 February 2012 - 10:38 AM
Posted 06 February 2012 - 11:06 AM
Posted 06 February 2012 - 12:58 PM
The freezing is the SDS freezing, it will also do it if you cool it below about 15 deg C.
yeah, I prepared fresh and it worked fine this time. It must have been NaOH. though I have heard SDS solution also expires after a while. I have also noticed when I put solution 2 on ice it freezes fast.