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How to Dilute Cell Lysate for Luciferase Assay

luciferase lysate dilution lysis assay

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#1 apc_nyca

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Posted 04 February 2012 - 09:27 AM

Hi, I am wondering what I should use to dilute the lysate that came with my Luciferase kit. Do I use TE buffer? Or DI water?
Thanks
A.P.C.

#2 scolix

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Posted 04 February 2012 - 10:44 AM

You could use something from the or check the kit manual. I have used PBS, but do you need to dilute the samples?

#3 KevinK

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Posted 06 February 2012 - 10:14 AM

apx_nyca,
I would strongly recommend that you dilute the lysate with additional lysis buffer. This will keep the amount of the diluent components the same regardless of dilution factor of the sample. Addition of other buffers or components could resutl in the addition of a variable amount of an inhibitor or other factor that impacts the luciferase reaction. If you have any additional questions or concerns, please do not hesitate to contact Promega Technical Services.

Kind regards,
Kevin
Promega Corporation
Madison, WI





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