I have been extracting proteins from rat brain, heart and kidney for use as positive controls on my Western Blots. I have precipitated the protein from the supernatant using TCA and the resulting pellets are washed with ether.
The problem is that some of the pellets will not solubilise into 1:1 sodium carbonate:DTT even under sonication. Does anyone have any ideas as to how I can get these proteins into solution?
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Protein pellet solubilisation
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