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Protein pellet solubilisation


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#1 floyd78

floyd78

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Posted 02 May 2003 - 01:30 PM

I have been extracting proteins from rat brain, heart and kidney for use as positive controls on my Western Blots. I have precipitated the protein from the supernatant using TCA and the resulting pellets are washed with ether.
The problem is that some of the pellets will not solubilise into 1:1 sodium carbonate:DTT even under sonication. Does anyone have any ideas as to how I can get these proteins into solution?

Thanks

Rachel




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