10 mM Na Acetate buffer preparation
Posted 03 February 2012 - 06:49 PM
Can anyone guide me on how this 10 mM buffer is prepared or on its calculation.
What currently I do is the old method that we have been following i.,e use 0.123 mg Na Acetate trihydrate + 0.476 µl Gl. Acetic acid, adjust pH to 4.0 using 5 N NaOH. But we do not know how these values have been arrived at and if this calculation is correct.
Please guide me with this regards.
Posted 03 February 2012 - 10:11 PM
try these two sites:
it is easy,
Posted 09 February 2012 - 02:03 PM
you can use sodium acetate solid but if you have to adjust the pH down then you should not use acetic acid or you will increase the buffer molarity. use hcl if your final buffer contains nacl or kcl.
the method you cite is probably calculated to give the proper final acetate concentration.
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