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Suggestions on hard to express proteins in E. coli


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#1 pDNA

pDNA

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Posted 03 February 2012 - 09:13 AM

Dear people,

since we are currently looking for proteins to challenge our protein expression platform i would be happy to hear your thoughts on proteins that are hard or nearly impossible to express in E. coli.

I would be happy to hear your thoughts on proteins that are hard to express (low yields, toxic genes) ...and where easy and fast downstream analytics (enzyme assay, ELISA, whatever) are available. I'm sure hundred of people here in this forum have wrecked their heads on expression of proteins ...your input would be highly appreciated.

Many thanks in advance!

Best regards,
p




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