Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo

Identification of a novel virus


  • Please log in to reply
No replies to this topic

#1 Paul

Paul

    member

  • Members
  • Pip
  • 3 posts
0
Neutral

Posted 02 May 2003 - 09:30 AM

I am currently trying to identify a putative novel virus in cerebral tissues of canines displaying severe neurodegenerative signs. I have eliminated all known agents as the cause. I wish to perform representational difference analysis (RDA) on both disease and normal canine cerebral tissues to identify a putative viral pathogen which is non-cultureable. If the pathogen is an RNA virus then I need to perform double strand cDNA synthesis on total RNA using random primers and not Oligo dT primers as this would pick up mRNA only.

1. Can random primers be used in RDA?
2. Does anyone know of an easier way to try and amplify a novel viral agent?

Many thanks for your help,
Regards,
Paul[B]




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.