problem in making a proper smear for nile blue staining of an unknown yeast
Posted 02 February 2012 - 03:33 AM
Posted 02 February 2012 - 12:31 PM
Posted 10 February 2012 - 02:03 AM
As for the cells fluorescing, then it's likely a positive for PHB. Then again, Nile blue is lipophilic so you know what this means: GC or HPLC. But by an large, a positive screening with Nile blue usually gives a positive results later on when you're confirming it by chromatography.
What I did with for staining during the time when the microscope wasn't available was I followed the methods of Kitamura and Doi (http://www.springerl...9612600770q703/).